Density gradient centrifugation studies on rabies virus.

Neurath, A. Robert (The Wistar Institute, Philadelphia, Pa.), Tadeusz J. Wiktor, and Hilary Koprowski. Density gradient centrifugation studies on rabies virus. J. Bacteriol. 92:102-106. 1966.-Cesium chloride density gradient centrifugation of rabies virus revealed a heterogeneous population of infectious virus particles, the majority of which showed a density of 1.20 g/ml. From results obtained by rate zonal centrifugation in preformed sucrose gradients, it was possible to calculate a sedimentation coefficient of about 600S for rabies virus. Sedimentation coefficients of about 23S and 10S were calculated for two soluble rabies antigens present in infected tissue-culture fluids, and they showed a density of 1.26 g/ml in cesium chloride solutions.